Page: 08-12. Development of method for the determination of aflatoxin m1 in raw milk and milk powder by Liquid Chromatography Tandem Mass Spectrometry (LCMSMS).

M. MATIUR RAHIM¹*, M. TARIQUL HASSAN¹, M. NURUL HUDA BHUIYAN¹, MAKSUDA BEGUM¹ and M. ANWAR HOSSAIN²

 ¹Food Toxicology Research Laboratory, Institute of Food Science & Technology (IFST), Bangladesh Council for Scientific and Industrial Research (BCSIR), Dhaka-1205 and ²Process Engineer, Chemicals Incorporated, Baytown, Texas, USA. *Corresponding author’s Email: matiurrahim@yahoo.com

ABSTRACT

The study was conducted during 2011-2013 at the Food Toxicology Laboratory of  IFST, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh under an R&D project entitled “Occurrence of aflatoxin M1 in milk and dairy products available in the markets of  Bangladesh”.  Aflatoxin M1(AFM1) is a hepatocarcinogen found in milk of animals that have consumed feeds contaminated with aflatoxin B1(AFB1), the most important toxic metabolite produced by storage fungi of the genus Aspergillus, particularly Aspergillus flavus and Aspergillus parasiticus. This procedure describes the extraction of Aflatoxin M1 from milk samples using a Phenyl solid phase extraction (SPE) procedure followed by liquid chromatography tandem mass spectrometry (LCMSMS) analysis. The method allows for superior clean up and selective detection with a detection limit of 12.50 ng/L.

Keywords:  Aflatoxin M1, Raw milk, Powder milk, SPE Clean-up and LCMSMS method.

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