A.H. M. SHAMIMUZZAMAN1, S. AHMED2*, M. PURKAYASTHA3, M. ASIRUDDIN4 and M. H. LIAKOT5
1=Abul Hasan Mohammed Shamimuzzaman, ULO, Alomdanga, Chuadanga; Department of Livestock Services, GOB, 2Dr. Sultan Ahmed, Associate Professor, Department of Microbiology & Immunology, Sylhet Agricultural University, Sylhet, 3Mousumi Purkayastha, Assistant Professor, Department of Microbiology & Immunology, Sylhet Agricultural University, Sylhet, 4Moammed Asiruddin, ULO, Kulaura, Moulvibazar; Department of Livestock Services, GOB and 5Mohammad Hossen Liakot ULO, Leave Reserve; Department of Livestock Services, GOB. *Corresponding author’s Email: drsultanahmed@yahoo.com.
ABSTRACT
A study was conducted at the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh (from July’99 to December ‘99) to isolate and identify the causal organism of enterotoxaemia (Clostridium perfringens), experimental production of infection in kids and to determine antibiogram pattern of C. perfringens. Samples were collected from suspected goats, main postmortem changes observed, were extensive hemorrhages and ulceration on the mucosa of small intestine; intestines filled with bloody fluid and fibrin clot; gelatinous fluid in the abdominal cavity and pericardial sac; rumen moderately congested; swollen, soft and hemorrhagic kidneys; enlarged mesenteric lymph nodes, congestion and consolidation of lungs. Samples were collected aseptically and cultured in blood agar and cooked meat broth, both anaerobically and aerobically. Sugar fermentation and others bio-chemical tests were conducted. Based on Gram’s staining, cultural characteristics, bio-chemical and sugar fermentation tests; Gram positive short thick anaerobic bacilli were identified. The reactions were similar or identical to Clostridium perfringens. Mice were inoculated with the toxins separated and prepared from broth culture showed the signs of toxemia and died within 20 hours of inoculation. For dermonecrotic test, toxins were injected in the skin of kids and purplish necrotic areas were observed. The signs in experimentally inoculated kids were typical and they died within 24 hours. The necropsy findings; were hemorrhagic enteritis, congested liver and lungs, soft hemorrhagic kidneys, congestion-petechiation and necrosis in the intestinal mucosa. Antibiotics sensitivity test revealed that, the isolated bacteria were sensitive to eight antimicrobial agents but resistant to Metronidiazole and Trisulpha that might be due to widely use of these two antimicrobials in human and veterinary practice in Bangladesh.
Keywords: Clostridium perfringens, Goat enterotoxaemia, Antibiogram and Enterotoxaemia.