M. A. HOQUE1*, M. G. RASUL2, M. A. K. MIAN2, T. HOSSAIN3 and M. M. RAHMAN4
1Associate Professor, Department of Agricultural Botany, Sher-e-Bangla Agricultural University, Dhaka, 2Professor, Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 3Professor, Department of Crop Botany, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 4Professor, Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh. *Corresponding author’s Email: ashadipu@yahoo.com
ABSTRACT
The experiments were conducted at the Research Fram and Advanced Plant Breeding Laboratory, Department of Genetics and Plant Breeding with the collaboration of Tissue Culture Laboratory, Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh during October 2010 to March 2011 and October 2011 to March 2012. GA3 application for the viable male organ development differed significantly in different treatments (T1= GA3 400 ppm, T2= GA3 600ppm, T3= GA3 800 ppm, T4 =GA3 1000ppm and T5= GA3 1200ppm). The highest number of viable pollen was found in the treatment T5= 1200 ppm (86.90%) followed by the treatment T4=1000 ppm (8.4%). There was significant difference for percent of callus formation from embryo in the treatments of days after pollination. The highest number of callus formation was found in the treatment 30 DAP (25%) followed by 35 DAP (15%) and 25 DAP (6.67%). The maximum response of shoot development from callus was found in the treatment 30 DAP (16.67%). The interspecific hybrid required 42.00 and 45.67 for days to male and female flower, respectively where as their parent OP-50 required 89.33 and 91.67 days for those characters. The hybrid contained high dry matter (11.21%) than the parent OP-50 (9.69%) but less than the parent C. maxima (17.89%). Total soluble solid content of the hybrid was 12.53 percent where as the parent’s C. maxima and OP-50 contained 14.78% and 8.93% total soluble solid, respectively. The hybrid contained higher beta-carotene (0.378 mg/g) than their parents.
Keywords: GA3, Viable pollen, Embryo culture, Interspecific hybrid and Pumpkin.