M. K. AHMMED1*, M. FAISAL2, F. AHMMED3, M. R. ALI4 and M. N. AHSAN5
1=Mirja Kaizer Ahmmed, Lecturer, Department of Fishing and Post Harvest Technology, Faculty of Fisheries, Chittagong Veterinary and Animal Sciences University, Chittagong, 2Md. Faisal, Assistant Professor, Department of Fishing and Post Harvest Technology, Faculty of Fisheries, Chittagong Veterinary and Animal Sciences University, Chittagong, 3Fatema Ahmmed, MS Student, Fisheries and Marine Resource Technology, Khulna University, Khulna, 4Md. Rayhan Ali, Artemia Reference Center, University of Ghent, Belgium and 5Md. Nazmul Ahsan, Professor, Fisheries and Marine resource Technology, Khulna University, Khulna, Bangladesh.
*Corresponding author’s Email: mirjafmrt@gmail.com.
ABSTRACT
The study was conducted at the Discipline of Fisheries and Marine Resource Technology, Khulna University, Khulna to know the complete repertoire of Cysteine Cathepsins in Green puffer fish by using Tetraodon genome database through in silico cloning. Cysteine Cathepsins are papain-like Cysteine proteases normally localized in lysosomes. The genomic sequence for a potential Cathepsin gene in Green pufferfish was identified by searching the Tetraodon genome database by using the mature part of some mammalian and fish Cathepsin sequences. The amino acid sequences of some mammalian and fish Cathepsins were collected from the GenBank through National Center for Biotechnology Information (NCBI) website. Stretches of amino acid residues, which are highly conserved among Cathepsins from different species, were used as a query sequence. Complete gene structure predictions have been made by GeneScan. Finally, eight Cysteine Cathepsins were found in Green puffer fish. Each of their eight Tetraodon genes have homologous in other fish mammalian species. Even the relative length of pre, pro and mature domains of Tetraodon Cysteine Cathepsins were found almost equal to representative Cathepsins of other animals reported so far. With the identified Cysteine Cathepsins, multiple sequence comparison and phylogenetic tree construction were carried out and the result revealed that the Tetraodon Cysteine Cathepsins have close evolutionary relationship with the fish Cathepsins, especially with tilapia Cathepsins.
Keywords: Cysteine Cathepsins, Green puffer fish, In silico cloning and GeneScan.