MD. MIZANUR RAHMAN1, MAHBUBUL KABIR HIMEL2, SANZIDA MUBASSARA3, MD. FOKHRUL ISLAM4 and APARNA SHIL2
1Former MSc Student, 2Assistant Professor, 3Professor, Department of Botany, Jahangirnagar University, Savar, Dhaka, 4Assistant Professor, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh.
*Corresponding author’s Email: himelbotju@yahoo.com.
ABSTRACT
The study was conducted at theDepartment of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh duringthe period of 2011to 2013.Traditional medicine plays a significant role in healthcare across the world and especially in regions of Asia. Many of these natural remedies are plant-based and have led to modern drug discovery in the 19th century and hence the surge in research into the bioactivity of plant products. Leaf and bark samples of Artocarpuschaplashawere extracted with methanol andsubjected to screening as a potential source of antioxidants and antibacterial activity.Phenolic acid and flavonoid content was evaluated by Folin-Ciocalteu reagent and aluminium chloride colorimetry respectively. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) was used to assess free radical scavenging activity, whilst a method developed from antibiotic susceptibility testing was conducted to investigate antibacterial activity. Brine Shrimp lethality assay was performed to determine cytotoxicity. The bark extract contained higher levels of both polyphenols and free radical scavenging activity with a calculated IC50 of 26.21 μg/mL. Both leaf and bark extracts displayed moderate antibacterial activity, but were induced cytotoxicity. The results indicated that extracts from A. chaplasha do exhibit antioxidant properties; however in their current form they are not suitable as natural remedies due to potentially harmful effects. However, profiling of polyphenols found in A. chaplasha could lead to the discovery of novel antioxidant and/or antibacterial compounds.
Keywords: Artocarpuschaplasha, Antioxidant, DPPH, IC50, Cytotoxicity and Antimicrobial.