M. MAHMUDA KHATUN1, M.HAZRAT ALI2 and RHODORA.R. ALDEMITA3
1=Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh, 2Rice Farming Systems Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh and 3Philippines Rice Research Institute, Maligaya, Science City of Munoz, Eueva Ecija, Philippines
ABSTRACT
The experiment was carried out in the Rice Transformation Laboratory of the Philippine Rice Research Institute, Maligaya, Science city of Munoz, Nueva Ecija, Philippines during 2001 to determine the transformation efficiency with the increased of acetosyrinogone using calli derived from seeds. A new plant type Lx 278 was used which was developed from a cross between Japonica and Indica rice varieties. Mature seed scutellar calli were inoculated with Agrobactacterium tumefaciens strain LBBA 4404 carrying a plasmid pTOK233 containing genes for b–glucuronidase (GUS) and hyrgomycin resistance (HygR). Hygromycin (30mg/l) was used as selectable agent. Inclusion of increased concentration (40 µl) of acetosyringone in the Agrobactacterium suspension proved to be better for successful transformation. Transformation efficiency was higher in the 40 µl of acetosyringone in both gus positive calli (3.33%) as well as hygromycin resistant calli (82.05%) compared to the lower concentration of acetosyringone.
Keywords: Transformation, Rice, Agrobacterium tumefaciens and acetosyringone.